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Mouse phospho-Insulin Receptor, β Subunit(pTyr1162/1163) ELISA Kit
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ELK0766
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48 T $320.00
96 T $458.00
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OverviewPublications(2)

Product name: Mouse phospho-Insulin Receptor, β Subunit(pTyr1162/1163) ELISA Kit
Reactivity: Mouse
Alternative Names: p-IR
Assay Type: Sandwich
Sensitivity: 0.68 U/mL
Standard: 100 U/mL
Detection Range: 1.57-100 U/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Endocrinology;Autoimmunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IR (Phospho) [pY1162/1163]. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IR (Phospho) [pY1162/1163]. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IR (Phospho) [pY1162/1163], biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IR (Phospho) [pY1162/1163] in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (U/mL) OD Corrected OD
100.00 2.079 1.978
50.00 1.593 1.492
25.00 1.183 1.082
12.50 0.857 0.756
6.25 0.509 0.408
3.13 0.325 0.224
1.57 0.199 0.098
0.00 0.101 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IR (Phospho) [pY1162/1163] and the recovery rates were calculated by comparing the measured value to the expected amount of IR (Phospho) [pY1162/1163] in samples.
Matrix Recovery range Average
serum(n=5) 82-98% 90%
EDTA plasma(n=5) 82-94% 88%
Heparin plasma(n=5) 80-97% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IR (Phospho) [pY1162/1163] and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-97% 84-99% 95-102% 88-103%
EDTA plasma(n=5) 95-104% 82-96% 92-103% 90-99%
Heparin plasma(n=5) 89-97% 85-94% 79-96% 85-99%