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Cattle anti-HAV IgG(anti-Hepatitis A antibody IgG) ELISA Kit
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ELK0762
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Cattle anti-HAV IgG(anti-Hepatitis A antibody IgG) ELISA Kit
Reactivity: Cattle
Alternative Names: Hepatitis A virus IgG
Assay Type: Sandwich
Sensitivity: 0.069 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle Anti-HAV IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle Anti-HAV IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle Anti-HAV IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle Anti-HAV IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.054 1.968
5.00 1.562 1.476
2.50 1.043 0.957
1.25 0.872 0.786
0.63 0.546 0.460
0.32 0.352 0.266
0.16 0.190 0.104
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-HAV IgG and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-HAV IgG in samples.
Matrix Recovery range Average
serum(n=5) 88-102% 95%
EDTA plasma(n=5) 78-92% 85%
Heparin plasma(n=5) 80-97% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-HAV IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-100% 79-91% 89-103% 82-95%
EDTA plasma(n=5) 87-98% 92-105% 95-107% 82-96%
Heparin plasma(n=5) 92-104% 86-98% 80-93% 89-97%