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Human Anti-Sulfatide (Anti-Sulfatide IgM antibody) ELISA Kit
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ELK0745
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48 T $320.00
96 T $458.00
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Overview

Product name: Human Anti-Sulfatide (Anti-Sulfatide IgM antibody) ELISA Kit
Reactivity: Human
Alternative Names: Anti-Sulfatide IgM antibody
Assay Type: Competitive Inhibition
Sensitivity: 0.049 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-Sulfatide. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-Sulfatide. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-Sulfatide in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 0.235
5.00 0.479
2.50 0.793
1.25 0.833
0.63 1.197
0.32 1.583
0.16 1.887
0.00 2.226

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-Sulfatide and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Sulfatide in samples.
Matrix Recovery range Average
serum(n=5) 96-107% 102%
EDTA plasma(n=5) 85-99% 92%
Heparin plasma(n=5) 83-95% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Sulfatide and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 98-107% 93-102% 80-93% 91-100%
EDTA plasma(n=5) 92-105% 85-101% 85-94% 87-96%
Heparin plasma(n=5) 84-94% 85-103% 94-105% 92-101%