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Human anti-SARS-CoV2(S-RBD) (Omicron,B.1.1.529) IgG ELISA Kit
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ELK0730
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48 T $320.00
96 T $458.00
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Overview

Product name: Human anti-SARS-CoV2(S-RBD) (Omicron,B.1.1.529) IgG ELISA Kit
Reactivity: Human
Alternative Names: anti-COVID-19;anti-SARS-CoV-2; anti-2019-nCoV
Assay Type: Sandwich
Sensitivity: 0.89 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample type: serum, plasma and biological fluids
Assay length: 3.5h
Research Area: Infectious Diseases
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human anti-SARS-CoV2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human anti-SARS-CoV2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human anti-SARS-CoV2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human anti-SARS-CoV2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.168 2.073
50.00 1.636 1.541
25.00 1.224 1.129
12.50 0.862 0.767
6.25 0.527 0.432
3.13 0.379 0.284
1.57 0.236 0.141
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant anti-SARS-CoV2 and the recovery rates were calculated by comparing the measured value to the expected amount of anti-SARS-CoV2 in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 86-99% 92%
Heparin plasma(n=5) 83-95% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti-SARS-CoV2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-103% 79-92% 86-99% 84-97%
EDTA plasma(n=5) 87-98% 92-105% 95-107% 82-96%
Heparin plasma(n=5) 86-105% 84-98% 95-102% 88-106%