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Human miRNA-210 ELISA Kit
ELK0726
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48 T $320.00
96 T $458.00
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Overview

Product name: Human miRNA-210 ELISA Kit
Reactivity: Human
Alternative Names: microRNA-210
Assay Type: Sandwich
Sensitivity: 11.97 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human miRNA-210. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human miRNA-210. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human miRNA-210, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human miRNA-210 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 2.054 1.971
1000.00 1.507 1.424
500.00 1.128 1.045
250.00 0.726 0.643
125.00 0.451 0.368
62.50 0.361 0.278
31.25 0.174 0.091
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant miRNA-210 and the recovery rates were calculated by comparing the measured value to the expected amount of miRNA-210 in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 86-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of miRNA-210 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-102% 96-105% 85-93% 89-97%
EDTA plasma(n=5) 82-96% 85-96% 87-101% 89-97%
Heparin plasma(n=5) 95-102% 89-96% 87-98% 85-93%