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Human Rock2(Rho Associated Coiled Coil Containing Protein Kinase 2) ELISA Kit
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ELK0700
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48 T $320.00
96 T $458.00
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Overview

Product name: Human Rock2(Rho Associated Coiled Coil Containing Protein Kinase 2) ELISA Kit
Reactivity: Human
Alternative Names: ROCK-II
Assay Type: Sandwich
Sensitivity: 0.13 ng/mL
Standard: 20 ng/ml
Detection Range: 0.32-20 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Uniprot ID: O75116
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Rock-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Rock-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Rock-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Rock-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.119 1.980
10.00 1.706 1.567
5.00 1.276 1.137
2.50 0.876 0.737
1.25 0.591 0.452
0.63 0.379 0.240
0.32 0.241 0.102
0.00 0.139 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Rock-2 and the recovery rates were calculated by comparing the measured value to the expected amount of Rock-2 in samples.
Matrix Recovery range Average
serum(n=5) 91-105% 98%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 84-97% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rock-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 86-92% 79-103% 89-97%
EDTA plasma(n=5) 85-94% 97-106% 79-91% 90-99%
Heparin plasma(n=5) 85-102% 81-96% 87-105% 83-92%