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Human Rock1(Rho-associated protein kinase 1) ELISA Kit
ELK0699
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human Rock1(Rho-associated protein kinase 1) ELISA Kit
Reactivity: Human
Alternative Names: P160ROCK; ROCK-I
Assay Type: Sandwich
Sensitivity: 0.15 ng/mL
Standard: 20 ng/ml
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;
Uniprot ID: Q13464
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Rock-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Rock-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Rock-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Rock-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 1.996 1.887
10.00 1.647 1.538
5.00 1.106 0.997
2.50 0.821 0.712
1.25 0.538 0.429
0.63 0.368 0.259
0.32 0.233 0.124
0.00 0.109 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Rock-1 and the recovery rates were calculated by comparing the measured value to the expected amount of Rock-1 in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 80-97% 88%
Heparin plasma(n=5) 87-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rock-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-99% 86-92% 89-103% 97-105%
EDTA plasma(n=5) 95-104% 87-96% 82-96% 89-103%
Heparin plasma(n=5) 85-92% 89-96% 90-98% 87-98%