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Mouse Anti-IAA(Anti-Insulin Autoantibody) ELISA Kit
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ELK0650
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Mouse Anti-IAA(Anti-Insulin Autoantibody) ELISA Kit
Reactivity: Mouse
Alternative Names: Anti-Insulin; Antiinsulin; Insulin Antibodies
Assay Type: Sandwich
Sensitivity: 0.32 ng/mL
Standard: 50 ng/mL
Detection Range: 0.78-50 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;Endocrinology;Autoimmunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Anti-IAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Anti-IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Anti-IAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Anti-IAA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
50.00 2.266 2.171
25.00 1.729 1.634
12.50 1.243 1.148
6.25 0.979 0.884
3.13 0.547 0.452
1.57 0.354 0.259
0.79 0.226 0.131
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-IAA and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-IAA in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 80-97% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-IAA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 79-92% 87-101% 82-90%
EDTA plasma(n=5) 80-94% 90-99% 87-103% 83-96%
Heparin plasma(n=5) 87-96% 86-95% 91-103% 87-93%