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Rabbit MyD88(Myeloid Differentiation Factor 88) ELISA Kit
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ELK0585
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Rabbit MyD88(Myeloid Differentiation Factor 88) ELISA Kit
Reactivity: Rabbit
Alternative Names: Myeloid Differentiation Primary Response Gene 88
Assay Type: Sandwich
Sensitivity: 20.4 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Pulmonology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit MyD88. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit MyD88. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit MyD88, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit MyD88 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
4000.00 2.045 1.977
2000.00 1.593 1.525
1000.00 1.213 1.145
500.00 0.726 0.658
250.00 0.589 0.521
125.00 0.329 0.261
62.50 0.247 0.179
0.00 0.068 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MyD88 and the recovery rates were calculated by comparing the measured value to the expected amount of MyD88 in samples.
Matrix Recovery range Average
serum(n=5) 78-90% 84%
EDTA plasma(n=5) 92-106% 99%
Heparin plasma(n=5) 90-105% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MyD88 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-94% 85-96% 78-90% 91-99%
EDTA plasma(n=5) 87-96% 85-97% 85-94% 87-96%
Heparin plasma(n=5) 92-101% 82-98% 89-97% 85-93%