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Human 6-K-PGF1α(6-keto-PGF1 alpha) ELISA Kit
ELK0571
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human 6-K-PGF1α(6-keto-PGF1 alpha) ELISA Kit
Reactivity: Human
Alternative Names: 6-keto-PGF1 alpha; 6ketoPGF1α
Assay Type: Competitive Inhibition
Sensitivity: 5.4 pg/mL
Standard: 800 pg/mL
Detection Range: 12.5-800 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2.5h
Research Area: Neuro science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 6-K-PGF1α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 6-K-PGF1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 6-K-PGF1α in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
800.00 0.199
400.00 0.397
200.00 0.722
100.00 0.833
50.00 1.169
25.00 1.585
12.50 1.762
0.00 2.256

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 6-K-PGF1α and the recovery rates were calculated by comparing the measured value to the expected amount of 6-K-PGF1α in samples.
Matrix Recovery range Average
serum(n=5) 92-105% 98%
EDTA plasma(n=5) 84-96% 90%
Heparin plasma(n=5) 80-95% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 6-K-PGF1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-93% 95-104% 82-96% 85-98%
EDTA plasma(n=5) 85-94% 87-96% 92-101% 82-98%
Heparin plasma(n=5) 85-94% 87-96% 82-90% 92-101%