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Human CA72-4(Carbonhydrate Antigen 72-4) ELISA Kit
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ELK0545
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Human CA72-4(Carbonhydrate Antigen 72-4) ELISA Kit
Reactivity: Human
Alternative Names: CA72-4
Assay Type: Sandwich
Sensitivity: 0.3 U/mL
Standard: 40 U/mL
Detection Range: 0.63-40 U/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA72-4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA72-4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA72-4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA72-4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (U/mL) OD Corrected OD
40.00 1.987 1.905
20.00 1.602 1.520
10.00 1.128 1.046
5.00 0.821 0.739
2.50 0.521 0.439
1.25 0.321 0.239
0.63 0.175 0.093
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CA72-4 and the recovery rates were calculated by comparing the measured value to the expected amount of CA72-4 in samples.
Matrix Recovery range Average
serum(n=5) 78-93% 85%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 89-103% 96%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA72-4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 78-92% 85-96% 93-102% 95-104%
EDTA plasma(n=5) 87-96% 85-98% 89-102% 85-98%
Heparin plasma(n=5) 87-98% 92-104% 82-96% 95-102%