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Plant γABA(Gamma-Aminobutyric Acid) ELISA Kit
ELK0526
Size： Price：
48 T $265.00
96 T $376.00
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Overview

Product name: Plant γABA(Gamma-Aminobutyric Acid) ELISA Kit
Reactivity: Plant
Alternative Names: 4-Aminobutyric Acid
Assay Type: Competitive Inhibition
Sensitivity: 15.3 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: plant tissues
Assay Length: 2.5h
Research Area: Signal transduction;Cardiovascular biology;Neuro science;Bone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Plant γABA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plant γABA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plant γABA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 0.155
1000.00 0.386
500.00 0.524
250.00 0.812
125.00 1.139
62.50 1.557
31.25 1.697
0.00 2.112

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant γABA and the recovery rates were calculated by comparing the measured value to the expected amount of γABA in samples.
Matrix Recovery range Average
serum(n=5) 86-99% 92%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 80-95% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of γABA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-94% 93-102% 87-96% 92-105%
EDTA plasma(n=5) 81-97% 88-102% 85-93% 83-97%
Heparin plasma(n=5) 90-99% 95-106% 87-101% 82-97%