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Human Anti-NMDAR(Anti-N-methyl-D-aspartic Acid Receptor Antibody) ELISA Kit
ELK0448
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48 T $320.00
96 T $458.00
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Overview

Product name: Human Anti-NMDAR(Anti-N-methyl-D-aspartic Acid Receptor Antibody) ELISA Kit
Reactivity: Human
Alternative Names: NMDARAb
Assay Type: Sandwich
Sensitivity: 0.65 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-NMDAR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-NMDAR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-NMDAR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-NMDAR in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.126 2.038
50.00 1.665 1.577
25.00 1.106 1.018
12.50 0.852 0.764
6.25 0.593 0.505
3.13 0.336 0.248
1.57 0.234 0.146
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-NMDAR and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-NMDAR in samples.
Matrix Recovery range Average
serum(n=5) 81-93% 87%
EDTA plasma(n=5) 82-94% 88%
Heparin plasma(n=5) 85-97% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-NMDAR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-102% 81-96% 86-93% 82-95%
EDTA plasma(n=5) 80-94% 90-99% 87-103% 83-96%
Heparin plasma(n=5) 91-99% 88-97% 88-104% 97-108%