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SA(Sialic Acid) ELISA Kit
ELK0424
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: SA(Sialic Acid) ELISA Kit
Reactivity: General
Alternative Names: SA
Assay Type: Competitive Inhibition
Sensitivity: 25.5 µg/mL
Standard: 5000 µg/mL
Detection Range: 78.13-5000 µg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 2.5h
Research Area: Signal transduction;Tumor immunity;Hepatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (µg/mL) OD Corrected OD
5000.00 0.175
2500.00 0.479
1250.00 0.767
625.00 0.967
312.50 1.112
156.25 1.511
78.13 1.871
0.00 2.115

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant SA and the recovery rates were calculated by comparing the measured value to the expected amount of SA in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 86-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 79-91% 96-105% 80-92% 91-103%
EDTA plasma(n=5) 92-104% 87-96% 89-101% 82-90%
Heparin plasma(n=5) 84-99% 83-101% 86-99% 93-101%