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Human NKX2-5(NK2 Homeobox 5) ELISA Kit
ELK0416
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human NKX2-5(NK2 Homeobox 5) ELISA Kit
Reactivity: Human
Alternative Names: Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E; CSX; NKX2.5; NKX2E
Assay Type: Sandwich
Sensitivity: 21.4 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction
Uniprot ID: P52952
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NKX2-5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKX2-5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NKX2-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NKX2-5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 1.954 1.873
2500.00 1.597 1.516
1250.00 1.121 1.040
625.00 0.882 0.801
312.50 0.595 0.514
156.25 0.315 0.234
78.13 0.215 0.134
0.00 0.081 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NKX2-5 and the recovery rates were calculated by comparing the measured value to the expected amount of NKX2-5 in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 86-99% 92%
Heparin plasma(n=5) 80-95% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NKX2-5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 82-98% 89-97% 85-93%
EDTA plasma(n=5) 85-97% 87-96% 82-95% 92-105%
Heparin plasma(n=5) 85-92% 79-96% 89-100% 85-96%