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Human ERK1/2(Extracellular Signal Regulated Kinase 1/2) ELISA Kit
ELK0372
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human ERK1/2(Extracellular Signal Regulated Kinase 1/2) ELISA Kit
Reactivity: Human
Alternative Names: ERK; ERK-2; ERK2; ERT1; MAPK2; NS13; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK
Assay Type: Sandwich
Sensitivity: 0.12 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ERK1/2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ERK1/2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ERK1/2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ERK1/2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.075 1.979
10.00 1.562 1.466
5.00 1.114 1.018
2.50 0.682 0.586
1.25 0.529 0.433
0.63 0.236 0.140
0.32 0.186 0.090
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ERK1/2 and the recovery rates were calculated by comparing the measured value to the expected amount of ERK1/2 in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 81-95% 88%
Heparin plasma(n=5) 83-95% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ERK1/2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 79-91% 96-105% 80-92% 91-103%
EDTA plasma(n=5) 88-96% 86-95% 90-103% 87-90%
Heparin plasma(n=5) 87-94% 85-96% 92-102% 81-90%