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Dog β2-MG(Beta-2-Microglobulin) ELISA Kit
ELK0336
Size： Price：
48 T $265.00
96 T $376.00
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Overview

Product name: Dog β2-MG(Beta-2-Microglobulin) ELISA Kit
Reactivity: Dog
Alternative Names: Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin
Assay Type: Sandwich
Sensitivity: 0.23 µg/mL
Standard: 50 µg/mL
Detection Range: 0.78-50 µg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Infection immunity;Kidney biomarker;
Uniprot ID: P19341
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog β2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog β2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog β2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog β2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (µg/mL) OD Corrected OD
50.00 2.035 1.946
25.00 1.566 1.477
12.50 1.052 0.963
6.25 0.866 0.777
3.13 0.625 0.536
1.57 0.315 0.226
0.79 0.168 0.079
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant β2-MG and the recovery rates were calculated by comparing the measured value to the expected amount of β2-MG in samples.
Matrix Recovery range Average
serum(n=5) 80-92% 86%
EDTA plasma(n=5) 88-102% 95%
Heparin plasma(n=5) 90-105% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of β2-MG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-96% 87-101% 89-102% 85-98%
EDTA plasma(n=5) 85-105% 87-98% 91-102% 86-102%
Heparin plasma(n=5) 86-93% 91-105% 87-99% 99-105%