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Mouse LAB7-2(B-Lymphocyte Activation Antigen B7-2) ELISA Kit
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ELK0306
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Mouse LAB7-2(B-Lymphocyte Activation Antigen B7-2) ELISA Kit
Reactivity: Mouse
Alternative Names: CD86; B7-2; B70; FUN-1; CD28LG2; CD28 Antigen Ligand 2,B7-2 Antigen; CTLA-4 counter-receptor B7.2
Assay Type: Sandwich
Sensitivity: 0.048 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LAB7-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LAB7-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LAB7-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LAB7-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 1.978 1.883
5.00 1.636 1.541
2.50 1.135 1.040
1.25 0.775 0.680
0.63 0.487 0.392
0.32 0.322 0.227
0.16 0.238 0.143
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant LAB7-2 and the recovery rates were calculated by comparing the measured value to the expected amount of LAB7-2 in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 86-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LAB7-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 78-92% 85-96% 93-102% 95-104%
EDTA plasma(n=5) 88-104% 93-102% 82-97% 92-101%
Heparin plasma(n=5) 85-92% 80-96% 93-105% 90-99%