Products

Mouse PIEZO-1(Piezo-Type mMechanosensitive iIon Channel Component 1) ELISA Kit
ELK0282
Size： Price：
48 T $320.00
96 T $458.00
Manual MSDS Send email to us

Overview

Product name: Mouse PIEZO-1(Piezo-Type mMechanosensitive iIon Channel Component 1) ELISA Kit
Reactivity: Mouse
Alternative Names: PIEZO1; FAM38A; KIAA0233
Assay Type: Sandwich
Sensitivity: 22.3 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;
Uniprot ID: E2JF22
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PIEZO-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIEZO-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PIEZO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIEZO-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.368 2.287
2500.00 1.585 1.504
1250.00 1.115 1.034
625.00 0.892 0.811
312.50 0.563 0.482
156.25 0.368 0.287
78.13 0.218 0.137
0.00 0.081 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PIEZO-1 and the recovery rates were calculated by comparing the measured value to the expected amount of PIEZO-1 in samples.
Matrix Recovery range Average
serum(n=5) 86-99% 92%
EDTA plasma(n=5) 89-103% 96%
Heparin plasma(n=5) 83-97% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIEZO-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-102% 82-94% 85-96% 78-90%
EDTA plasma(n=5) 85-94% 97-106% 82-95% 93-105%
Heparin plasma(n=5) 85-92% 83-96% 78-98% 90-99%