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Human CA15-3(Carbonhydrate Antigen 15-3) ELISA Kit
ELK0245
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48 T $320.00
96 T $458.00
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Overview

Product name: Human CA15-3(Carbonhydrate Antigen 15-3) ELISA Kit
Reactivity: Human
Alternative Names: CA15-3
Assay Type: Sandwich
Sensitivity: 0.47 U/mL
Standard: 100 U/mL
Detection Range: 1.57-100 U/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA15-3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA15-3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA15-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA15-3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (U/mL) OD Corrected OD
100.00 2.126 2.040
50.00 1.613 1.527
25.00 1.226 1.140
12.50 0.826 0.740
6.25 0.576 0.490
3.13 0.373 0.287
1.57 0.135 0.049
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CA15-3 and the recovery rates were calculated by comparing the measured value to the expected amount of CA15-3 in samples.
Matrix Recovery range Average
serum(n=5) 81-95% 88%
EDTA plasma(n=5) 78-92% 85%
Heparin plasma(n=5) 88-106% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA15-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-96% 91-101% 87-98% 89-103%
EDTA plasma(n=5) 85-94% 87-96% 79-95% 95-102%
Heparin plasma(n=5) 85-92% 79-96% 87-103% 90-99%