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Rat DNM1L(Dynamin 1 Like Protein) ELISA Kit
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ELK0215
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Rat DNM1L(Dynamin 1 Like Protein) ELISA Kit
Reactivity: Rat
Alternative Names: DRP1; DLP1; DVLP; DYMPLE; HDYNIV; VPS1; Dynamin-related protein 1; Dnm1p/Vps1p-like protein; Dynamin family member proline-rich carboxyl-terminal domain less
Assay Type: Sandwich
Sensitivity: 0.064 ng/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;
Uniprot ID: O35303
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DNM1L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DNM1L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DNM1L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DNM1L in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.157 2.076
2500.00 1.657 1.576
1250.00 1.174 1.093
625.00 0.873 0.792
312.50 0.528 0.447
156.25 0.423 0.342
78.13 0.248 0.167
0.00 0.081 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant DNM1L and the recovery rates were calculated by comparing the measured value to the expected amount of DNM1L in samples.
Matrix Recovery range Average
serum(n=5) 94-107% 100%
EDTA plasma(n=5) 94-105% 98%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DNM1L and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-102% 86-92% 91-103% 89-97%
EDTA plasma(n=5) 88-103% 83-94% 92-105% 89-97%
Heparin plasma(n=5) 85-97% 87-96% 82-95% 92-105%