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Rat CA19-9(Carbohydrate Antigen 19-9) Microsample ELISA Kit
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ELK0162MS
Size： Price：
48 T $265.00
96 T $376.00
96 T*5 $1598.00
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Overview

Product name: Rat CA19-9(Carbohydrate Antigen 19-9) Microsample ELISA Kit
Reactivity: Rat
Alternative Names: sialylated Lewis(a)Antigen; Cancer Antigen 19-9
Assay Type: Sandwich
Sensitivity: 0.3 U/mL
Standard: 50 U/mL
Detection Range: 0.79-50 U/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Area: Tumor immunity;Hepatology;Gastroenterology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CA19-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CA19-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CA19-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CA19-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (U/mL) OD Corrected OD
50.00 2.250 2.169
25.00 1.665 1.584
12.50 1.285 1.204
6.25 0.817 0.736
3.13 0.615 0.534
1.56 0.325 0.244
0.78 0.178 0.097
0.00 0.081 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CA19-9 and the recovery rates were calculated by comparing the measured value to the expected amount of CA19-9 in samples.
Matrix Recovery range Average
serum(n=5) 90-112% 101%
EDTA plasma(n=5) 95-118% 107%
Heparin plasma(n=5) 94-101% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA19-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-116% 85-94% 93-107% 88-109%
EDTA plasma(n=5) 84-104% 87-116% 83-99% 88-102%
Heparin plasma(n=5) 88-103% 92-104% 92-108% 81-91%

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