|Product name:||EasyStep Human PSA(ProstateSpecific Antigen) ELISA Kit|
|Alternative Names:||KLK3; APS; KLK2A1; hK3; Kallikrein-3; Kallikrein-Related Peptidase 3; Gamma-seminoprotein|
|Detection Range:||0.31 ng/ml-20 ng/ml|
|Sample Type:||Serum, plasma|
|Research Area:||Tags & Cell Markers;Neuroscience;Signal Transduction;Cancer|
|Test principle:||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSA, and the Human PSA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PSA . After TMB substrate solution is added, only those wells that contain Human PSA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSA in the samples is then determined by comparing the OD of the samples to the standard curve|
|Concentration (ng/mL)||OD||Corrected OD|
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.