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Human F-actin(Filamentous Actin) Microsample ELISA Kit
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ELK0113MS
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Human F-actin(Filamentous Actin) Microsample ELISA Kit
Reactivity: Human
Alternative Names: Fibrous Actin
Assay Type: Sandwich
Sensitivity: 0.18 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: Serum, plasma, tissue homogenates
Assay Length: 3.5h
Research Area: Signal transduction;Developmental science;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-actin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-actin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-actin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-actin in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
40.00 2.517 2.440
20.00 1.671 1.594
10.00 1.094 1.017
5.00 0.762 0.685
2.50 0.609 0.532
1.25 0.381 0.304
0.63 0.214 0.137
0.00 0.077 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant F-actin and the recovery rates were calculated by comparing the measured value to the expected amount of F-actin in samples.
Matrix Recovery range Average
serum(n=5) 94-114% 104%
EDTA plasma(n=5) 91-123% 107%
Heparin plasma(n=5) 91-118% 105%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F-actin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-109% 90-117% 83-95% 82-103%
EDTA plasma(n=5) 91-103% 85-110% 81-86% 86-97%
Heparin plasma(n=5) 82-104% 91-106% 85-95% 85-113%

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