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Rat MuRF1(muscle-specific RING-finger protein 1) ELISA Kit
ELK9868
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat MuRF1(muscle-specific RING-finger protein 1) ELISA Kit
Reactivity: Rat
Alternative Names: MuRF1 ; IRF ; RNF28 ; SMRZ ; TRIM63 ; IRFMURF2 ; Iris RING finger protein ; MuRF1 ; MURF1 ; MuRF-1 ; muscle specific ring finger protein 2 ; Muscle-specific RING finger protein 1 ; RING finger protein 28FLJ32380 ; RNF28E3 ubiquitin-protein ligase TRIM63 ; SMRZMURF-1 ; Striated muscle RING zinc finger protein ; tripartite motif containing 63 ; tripartite motif-containing 63 ; Tripartite motif-containing protein 63
Assay Type: Sandwich
Sensitivity: 6.9 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Cell Biology
Uniprot ID: Q91Z63
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MuRF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MuRF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MuRF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MuRF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.054 1.971
500.00 1.537 1.454
250.00 1.113 1.030
125.00 0.826 0.743
62.50 0.527 0.444
31.25 0.336 0.253
15.63 0.252 0.169
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MuRF1 and the recovery rates were calculated by comparing the measured value to the expected amount of MuRF1 in samples.
Matrix Recovery range Average
serum(n=5) 81-95% 88%
EDTA plasma(n=5) 90-103% 96%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MuRF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-103% 82-96% 87-105% 90-99%
EDTA plasma(n=5) 79-93% 86-92% 84-98% 89-102%
Heparin plasma(n=5) 88-97% 93-103% 94-106% 91-101%