Products

Rabbit FPA(Fibrinopeptide A) ELISA Kit
ELK9698
Size： Price：
48 T $320.00
96 T $458.00
Download ① Send email to us

Overview

Product name: Rabbit FPA(Fibrinopeptide A) ELISA Kit
Reactivity: Rabbit
Alternative Names: FP-A
Assay Type: Competitive Inhibition
Sensitivity: 136.7 pg/mL
Standard: 30000 pg/mL
Detection Range: 468.75-30000 pg/mL
Sample Type: Plasma.
Assay Length: 2h
Research Area: Signal transduction;Hematology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rabbit FPA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit FPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit FPA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
30000.00 0.227
15000.00 0.411
7500.00 0.651
3750.00 0.856
1875.00 1.157
937.50 1.592
468.75 1.755
0.00 2.213

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant FPA and the recovery rates were calculated by comparing the measured value to the expected amount of FPA in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 88-102% 95%
Heparin plasma(n=5) 82-97% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FPA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 80-101% 87-96% 96-105% 95-103%
EDTA plasma(n=5) 92-101% 87-98% 85-92% 79-96%
Heparin plasma(n=5) 85-94% 79-96% 98-105% 82-93%