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F-E3(Free Estriol) ELISA Kit
ELK9646
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48 T $265.00
96 T $376.00
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Overview

Product name: F-E3(Free Estriol) ELISA Kit
Reactivity: General
Alternative Names: F-E3; Free Estriol
Assay Type: Competitive Inhibition
Sensitivity: 0.218 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with F-E3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to F-E3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F-E3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
40.00 0.215
20.00 0.310
10.00 0.596
5.00 0.895
2.50 1.169
1.25 1.585
0.63 1.869
0.00 2.157

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant F-E3 and the recovery rates were calculated by comparing the measured value to the expected amount of F-E3 in samples.
Matrix Recovery range Average
serum(n=5) 83-95% 89%
EDTA plasma(n=5) 79-93% 86%
Heparin plasma(n=5) 92-105% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F-E3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-93% 91-103% 87-98% 92-99%
EDTA plasma(n=5) 89-99% 83-96% 98-104% 88-101%
Heparin plasma(n=5) 87-96% 92-105% 89-102% 92-101%