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Rat Cys-C(Cystatin C) ELISA Kit
ELK9629
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat Cys-C(Cystatin C) ELISA Kit
Reactivity: Rat
Alternative Names: CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin
Assay Type: Sandwich
Sensitivity: 0.10 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Stem Cells;Cardiovascular;Cell Biology;Tags & Cell Markers;
Uniprot ID: P14841
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.096 2.009
5.00 1.686 1.599
2.50 1.120 1.033
1.25 0.828 0.741
0.63 0.567 0.480
0.32 0.318 0.231
0.16 0.248 0.161
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.
Matrix Recovery range Average
serum(n=5) 88-102% 95%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 79-93% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 81-93% 87-101% 96-105% 96-105%
EDTA plasma(n=5) 88-96% 87-98% 93-101% 91-99%
Heparin plasma(n=5) 85-97% 93-101% 87-98% 79-96%