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Human PDZRN3(PDZ domain containing ring finger 3) ELISA Kit
ELK9138
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48 T $265.00
96 T $376.00
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Overview

Product name: Human PDZRN3(PDZ domain containing ring finger 3) ELISA Kit
Reactivity: Human
Alternative Names: E3 ubiquitin-protein ligase PDZRN3; KIAA1095; Ligand of Numb protein X 3; likely ortholog of mouse semaF cytoplasmic domain associated protein 3; LNX3; PDZ domain containing ring finger 3; PDZ domain-containing RING finger protein 3; Pdzrn3; Protein SEMACAP3; PZRN3_HUMAN; SEMACAP3; Semaphorin cytoplasmic domain associated protein; Semaphorin cytoplasmic domain-associated protein 3; SEMCAP3
Assay Type: Sandwich
Sensitivity: 13.1 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.3-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Developmental Biology
Uniprot ID: Q9UPQ7
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDZRN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDZRN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDZRN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDZRN3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 2.075 1.987
1000.00 1.562 1.474
500.00 1.183 1.095
250.00 0.864 0.776
125.00 0.516 0.428
62.50 0.389 0.301
31.25 0.234 0.146
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PDZRN3 and the recovery rates were calculated by comparing the measured value to the expected amount of PDZRN3 in samples.
Matrix Recovery range Average
serum(n=5) 80-97% 88%
EDTA plasma(n=5) 80-92% 86%
Heparin plasma(n=5) 84-92% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDZRN3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 78-92% 83-96% 87-98% 95-102%
EDTA plasma(n=5) 86-97% 88-95% 92-101% 90-99%
Heparin plasma(n=5) 95-102% 82-90% 89-97% 87-98%