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Human PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit
ELK9034
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48 T $320.00
96 T $458.00
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Overview

Product name: Human PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit
Reactivity: Human
Alternative Names: PHOSPHATIDYLINOSITOL GLYCAN; Phosphatidylinositol glycan biosynthesis class F protein; Phosphatidylinositol-glycan biosynthesis class F protein; PIG F; PIG-F; PIGF; PIGF_HUMAN; PIGF1
Assay Type: Competitive Inhibition
Sensitivity: 6.2 ng/mL
Standard: 1000 ng/mL
Detection Range: 15.6-1000 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 2h
Research Area: Signal transduction;
Uniprot ID: Q07326
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human F protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 0.212
500.00 0.376
250.00 0.622
125.00 0.861
62.50 1.157
31.25 1.565
15.63 1.733
0.00 2.265

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant F and the recovery rates were calculated by comparing the measured value to the expected amount of F in samples.
Matrix Recovery range Average
serum(n=5) 80-97% 88%
EDTA plasma(n=5) 78-93% 85%
Heparin plasma(n=5) 78-96% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-96% 97-105% 95-107% 92-103%
EDTA plasma(n=5) 95-102% 85-96% 90-98% 82-93%
Heparin plasma(n=5) 95-107% 89-94% 81-94% 83-96%