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Human MT-RNR2 (Putative humanin peptide) ELISA Kit
ELK8996
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48 T $320.00
96 T $458.00
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Overview

Product name: Human MT-RNR2 (Putative humanin peptide) ELISA Kit
Reactivity: Human
Alternative Names: MT-RNR2; HN ; Humanin ; Humanin mitochondrial ; HNM
Assay Type: Sandwich
Sensitivity: 12.3 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates
Assay Length: 3.5h
Research Area: Others
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MT-RNR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MT-RNR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MT-RNR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MT-RNR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 2.172 2.080
1000.00 1.687 1.595
500.00 1.198 1.106
250.00 0.897 0.805
125.00 0.583 0.491
62.50 0.361 0.269
31.25 0.226 0.134
0.00 0.092 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MT-RNR2 and the recovery rates were calculated by comparing the measured value to the expected amount of MT-RNR2 in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 92-105% 98%
Heparin plasma(n=5) 84-96% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MT-RNR2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 79-96% 92-105% 91-99%
EDTA plasma(n=5) 81-93% 78-91% 87-98% 82-95%
Heparin plasma(n=5) 88-102% 84-97% 89-107% 85-92%