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Mouse Lptn/LTN/XCL1 (Lymphotactin) ELISA Kit
ELK8890
Size： Price：
48 T $412.00
96 T $588.00
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Overview

Product name: Mouse Lptn/LTN/XCL1 (Lymphotactin) ELISA Kit
Reactivity: Mouse
Alternative Names: Xcl1 ; Lptn ; Ltn ; Scyc1 ; Lymphotactin ; C motif chemokine 1 ; Cytokine SCM-1 ; Lymphotaxin ; Small-inducible cytokine C1
Assay Type: Sandwich
Sensitivity: 3.27 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;Dermatology;
Uniprot ID: P47993
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Lptn/LTN/XCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Lptn/LTN/XCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Lptn/LTN/XCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Lptn/LTN/XCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
500.00 2.036 1.939
250.00 1.525 1.428
125.00 1.088 0.991
62.50 0.882 0.785
31.25 0.468 0.371
15.63 0.312 0.215
7.82 0.268 0.171
0.00 0.097 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Lptn/LTN/XCL1 and the recovery rates were calculated by comparing the measured value to the expected amount of Lptn/LTN/XCL1 in samples.
Matrix Recovery range Average
serum(n=5) 88-99% 93%
EDTA plasma(n=5) 86-98% 92%
Heparin plasma(n=5) 82-98% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lptn/LTN/XCL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-94% 80-92% 86-102% 87-103%
EDTA plasma(n=5) 85-93% 79-96% 93-106% 87-99%
Heparin plasma(n=5) 82-96% 85-96% 87-101% 89-97%