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Mouse COLEC11(collectin sub-family member 11) ELISA Kit
ELK8823
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Mouse COLEC11(collectin sub-family member 11) ELISA Kit
Reactivity: Mouse
Alternative Names: COLEC11 ELISA Kit; Collectin Sub-Family Member 11 ELISA Kit; Collectin-11 ELISA Kit; CL-K1 ELISA Kit; Dual specificity protein kinase CLK1 ELISA Kit; Protein tyrosine kinase STY ELISA Kit; STY ELISA Kit
Assay Type: Sandwich
Sensitivity: 15 pg/mL
Standard: 2500 pg/mL
Detection Range: 39.07-2500 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism;
Uniprot ID: Q3SXB8
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse COLEC11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse COLEC11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse COLEC11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse COLEC11 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2500.00 2.126 2.031
1250.00 1.542 1.447
625.00 1.112 1.017
312.50 0.907 0.812
156.25 0.457 0.362
78.13 0.305 0.210
39.07 0.265 0.170
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant COLEC11 and the recovery rates were calculated by comparing the measured value to the expected amount of COLEC11 in samples.
Matrix Recovery range Average
serum(n=5) 92-107% 99%
EDTA plasma(n=5) 78-92% 85%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COLEC11 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-98% 92-104% 82-96% 95-102%
EDTA plasma(n=5) 89-97% 95-106% 85-101% 87-102%
Heparin plasma(n=5) 84-98% 89-102% 82-96% 87-98%