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Dog Pro-ANP(Pro Atrial Natriuretic Peptide) ELISA Kit
ELK8732
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48 T $320.00
96 T $458.00
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Overview

Product name: Dog Pro-ANP(Pro Atrial Natriuretic Peptide) ELISA Kit
Reactivity: Dog
Alternative Names: NT-proANP; Pro Atrial Natriuretic Peptide
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology
Uniprot ID: P07499
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog Pro-ANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog Pro-ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog Pro-ANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog Pro-ANP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.121 2.036
5.00 1.657 1.572
2.50 1.282 1.197
1.25 0.852 0.767
0.63 0.614 0.529
0.32 0.393 0.308
0.16 0.189 0.104
0.00 0.085 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Pro-ANP and the recovery rates were calculated by comparing the measured value to the expected amount of Pro-ANP in samples.
Matrix Recovery range Average
serum(n=5) 80-92% 86%
EDTA plasma(n=5) 83-97% 90%
Heparin plasma(n=5) 86-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pro-ANP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 87-98% 85-92% 79-96%
EDTA plasma(n=5) 95-104% 82-93% 81-96% 83-94%
Heparin plasma(n=5) 95-102% 89-96% 87-98% 85-93%