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Cattle 17-OHP(17-Hydroxyprogesterone) ELISA Kit
ELK8686
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48 T $265.00
96 T $376.00
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Overview

Product name: Cattle 17-OHP(17-Hydroxyprogesterone) ELISA Kit
Reactivity: Cattle
Alternative Names: 17OHP; 17-OH Progesterone; 17-Alpha-Hydroxyprogesterone
Assay Type: Competitive Inhibition
Sensitivity: 87.3 pg/mL
Standard: 20000 pg/mL
Detection Range: 312.5-20000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle 17-OHP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle 17-OHP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle 17-OHP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
20000.00 0.199
10000.00 0.397
5000.00 0.612
2500.00 0.895
1250.00 1.236
625.00 1.585
312.50 1.775
0.00 2.028

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 17-OHP and the recovery rates were calculated by comparing the measured value to the expected amount of 17-OHP in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 96-107% 101%
Heparin plasma(n=5) 90-105% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 17-OHP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-94% 85-96% 82-96% 95-102%
EDTA plasma(n=5) 86-97% 90-98% 80-93% 92-105%
Heparin plasma(n=5) 86-99% 93-106% 89-97% 85-94%