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Human sTNF-R2(soluble tumor necrosis factor receptor 2) ELISA Kit
ELK8640
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48 T $412.00
96 T $588.00
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Overview

Product name: Human sTNF-R2(soluble tumor necrosis factor receptor 2) ELISA Kit
Reactivity: Human
Alternative Names: sTNF-R2; soluble tumor necrosis factor receptor 2
Assay Type: Sandwich
Sensitivity: 9.13 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Cancer
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sTNF-R2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sTNF-R2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sTNF-R2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sTNF-R2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 2.204 2.103
1000.00 1.532 1.431
500.00 1.128 1.027
250.00 0.812 0.711
125.00 0.561 0.460
62.50 0.351 0.250
31.25 0.222 0.121
0.00 0.101 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sTNF-R2 and the recovery rates were calculated by comparing the measured value to the expected amount of sTNF-R2 in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 80-97% 88%
Heparin plasma(n=5) 92-107% 99%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sTNF-R2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-97% 83-96% 95-103% 88-95%
EDTA plasma(n=5) 95-104% 97-106% 85-98% 81-92%
Heparin plasma(n=5) 87-103% 79-92% 86-99% 84-97%