Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 2.266 | 2.163 |
500.00 | 1.586 | 1.483 |
250.00 | 1.217 | 1.114 |
125.00 | 0.898 | 0.795 |
62.50 | 0.611 | 0.508 |
31.25 | 0.318 | 0.215 |
15.63 | 0.221 | 0.118 |
0.00 | 0.103 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-93% | 86% |
EDTA plasma(n=5) | 95-108% | 101% |
Heparin plasma(n=5) | 82-95% | 88% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 95-102% | 98-106% | 87-98% | 91-102% |
EDTA plasma(n=5) | 85-96% | 95-104% | 93-102% | 79-94% |
Heparin plasma(n=5) | 86-93% | 91-105% | 87-98% | 89-94% |