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Human GSP(Glycated Serum Protein) ELISA Kit
ELK8558
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48 T $320.00
96 T $458.00
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Overview

Product name: Human GSP(Glycated Serum Protein) ELISA Kit
Reactivity: Human
Alternative Names: GSP
Assay Type: Competitive Inhibition
Sensitivity: 10.19 nmol/mL
Standard: 2000 nmol/mL
Detection Range: 31.25-2000 nmol/mL
Sample Type: serum, plasma
Assay Length: 2h
Research Area: Diabetes
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GSP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GSP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GSP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (nmol/mL) OD Corrected OD
2000.00 0.215
1000.00 0.396
500.00 0.615
250.00 0.823
125.00 1.258
62.50 1.501
31.25 1.697
0.00 2.232

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant GSP and the recovery rates were calculated by comparing the measured value to the expected amount of GSP in samples.
Matrix Recovery range Average
serum(n=5) 88-102% 95%
EDTA plasma(n=5) 79-97% 88%
Heparin plasma(n=5) 83-95% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GSP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-100% 84-99% 87-99% 99-105%
EDTA plasma(n=5) 86-95% 93-103% 85-94% 87-101%
Heparin plasma(n=5) 93-104% 87-98% 85-92% 79-96%