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Human Muscarinic Acetylcholine Receptor (M-AChR) ELISA Kit
ELK8532
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48 T $320.00
96 T $458.00
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Overview

Product name: Human Muscarinic Acetylcholine Receptor (M-AChR) ELISA Kit
Reactivity: Human
Alternative Names: M-AChR
Assay Type: Sandwich
Sensitivity: 0.135 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Infection immunity;Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human M-AChR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human M-AChR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human M-AChR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human M-AChR in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.164 2.070
10.00 1.579 1.485
5.00 1.116 1.022
2.50 0.796 0.702
1.25 0.424 0.330
0.63 0.329 0.235
0.32 0.216 0.122
0.00 0.094 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant M-AChR and the recovery rates were calculated by comparing the measured value to the expected amount of M-AChR in samples.
Matrix Recovery range Average
serum(n=5) 78-92% 85%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 80-97% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of M-AChR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 90-99% 95-106% 87-101% 82-97%
EDTA plasma(n=5) 79-93% 89-97% 85-94% 87-101%
Heparin plasma(n=5) 86-94% 85-95% 89-99% 85-95%