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Mouse Pro-MMP-9(Pro-Matrix Metalloproteinase 9) ELISA Kit
ELK8500
Size： Price：
48 T $412.00
96 T $588.00
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Overview

Product name: Mouse Pro-MMP-9(Pro-Matrix Metalloproteinase 9) ELISA Kit
Reactivity: Mouse
Alternative Names: Pro-MMP-9
Assay Type: Sandwich
Sensitivity: 0.459 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Tumor immunity;Infection immunity;Cardiovascular biology;Hepatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Pro-MMP-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Pro-MMP-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Pro-MMP-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Pro-MMP-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
50.00 2.169 2.088
25.00 1.628 1.547
12.50 1.034 0.953
6.25 0.835 0.754
3.13 0.496 0.415
1.57 0.357 0.276
0.79 0.175 0.094
0.00 0.081 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Pro-MMP-9 and the recovery rates were calculated by comparing the measured value to the expected amount of Pro-MMP-9 in samples.
Matrix Recovery range Average
serum(n=5) 80-92% 86%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 88-102% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pro-MMP-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 87-99% 85-101% 79-90%
EDTA plasma(n=5) 86-97% 88-95% 92-101% 87-98%
Heparin plasma(n=5) 85-92% 79-96% 89-100% 85-96%