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Rat Pro-ANP(Pro Atrial Natriuretic Peptide) ELISA Kit
ELK8360
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat Pro-ANP(Pro Atrial Natriuretic Peptide) ELISA Kit
Reactivity: Rat
Alternative Names: Pro-ANP
Assay Type: Sandwich
Sensitivity: 15.6 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Uniprot ID: P01161
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Pro-ANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Pro-ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Pro-ANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Pro-ANP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
4000.00 2.207 2.121
2000.00 1.625 1.539
1000.00 1.135 1.049
500.00 0.863 0.777
250.00 0.529 0.443
125.00 0.325 0.239
62.50 0.249 0.163
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Pro-ANP and the recovery rates were calculated by comparing the measured value to the expected amount of Pro-ANP in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 81-95% 88%
Heparin plasma(n=5) 88-96% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pro-ANP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-102% 86-92% 91-103% 89-97%
EDTA plasma(n=5) 81-93% 86-98% 91-103% 82-95%
Heparin plasma(n=5) 87-95% 85-98% 82-97% 92-101%