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Rat TG-ab(anti-Thyroglobulin Ab) ELISA Kit
ELK8353
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat TG-ab(anti-Thyroglobulin Ab) ELISA Kit
Reactivity: Rat
Alternative Names: TG AB; TG-AB
Assay Type: Sandwich
Sensitivity: 0.64 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TG-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TG-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TG-Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TG-Ab in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.185 2.096
50.00 1.527 1.438
25.00 1.193 1.104
12.50 0.855 0.766
6.25 0.539 0.450
3.13 0.312 0.223
1.57 0.235 0.146
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TG-Ab and the recovery rates were calculated by comparing the measured value to the expected amount of TG-Ab in samples.
Matrix Recovery range Average
serum(n=5) 92-105% 98%
EDTA plasma(n=5) 83-95% 89%
Heparin plasma(n=5) 79-93% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TG-Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-96% 92-105% 89-102% 92-101%
EDTA plasma(n=5) 93-99% 87-101% 85-95% 85-96%
Heparin plasma(n=5) 87-95% 85-96% 92-102% 81-92%