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Pig IFABP/FABP2(Intestinal Fatty Acid Binding Protein) ELISA Kit
ELK8306
Size： Price：
48 T $412.00
96 T $588.00
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Overview

Product name: Pig IFABP/FABP2(Intestinal Fatty Acid Binding Protein) ELISA Kit
Reactivity: Pig
Alternative Names: FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein; FABP2; Fatty Acid Binding Protein 2, Intestinal
Assay Type: Sandwich
Sensitivity: 6.4 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Tumor immunity;Endocrinology;Cardiovascular biology;
Uniprot ID: Q45KW7
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig IFABP/FABP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IFABP/FABP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig IFABP/FABP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig IFABP/FABP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 1.846 1.748
500.00 1.494 1.396
250.00 1.075 0.977
125.00 0.782 0.684
62.50 0.509 0.411
31.25 0.325 0.227
15.63 0.261 0.163
0.00 0.098 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IFABP/FABP2 and the recovery rates were calculated by comparing the measured value to the expected amount of IFABP/FABP2 in samples.
Matrix Recovery range Average
serum(n=5) 80-93% 86%
EDTA plasma(n=5) 94-105% 98%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFABP/FABP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-102% 93-105% 91-99% 83-96%
EDTA plasma(n=5) 79-95% 96-105% 85-96% 90-99%
Heparin plasma(n=5) 85-97% 93-101% 97-103% 91-99%