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Human Anti-THSD7A(Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody) ELISA Kit
ELK8249
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48 T $320.00
96 T $458.00
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Overview

Product name: Human Anti-THSD7A(Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody) ELISA Kit
Reactivity: Human
Alternative Names: THS7A; Thsd7a
Assay Type: Competitive Inhibition
Sensitivity: 1.22 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2.5h
Research Area: Signal transduction;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-THSD7A protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-THSD7A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-THSD7A in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 0.197
100.00 0.397
50.00 0.546
25.00 0.833
12.50 1.169
6.25 1.589
3.13 1.715
0.00 2.213

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-THSD7A and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-THSD7A in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 87-95% 91%
Heparin plasma(n=5) 78-93% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-THSD7A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 91-103% 86-92% 89-97%
EDTA plasma(n=5) 85-94% 87-96% 89-101% 82-90%
Heparin plasma(n=5) 87-101% 87-96% 82-93% 89-97%