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Rat MOTS-c(Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit
ELK8233
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat MOTS-c(Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit
Reactivity: Rat
Alternative Names: MOTS-c
Assay Type: Competitive Inhibition
Sensitivity: 0.94 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Developmental science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MOTS-c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MOTS-c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MOTS-c in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 0.185
100.00 0.413
50.00 0.596
25.00 0.997
12.50 1.197
6.25 1.522
3.13 1.889
0.00 2.101

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MOTS-c and the recovery rates were calculated by comparing the measured value to the expected amount of MOTS-c in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 78-90% 84%
Heparin plasma(n=5) 89-107% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MOTS-c and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-102% 86-92% 91-103% 89-97%
EDTA plasma(n=5) 95-102% 87-101% 82-91% 85-97%
Heparin plasma(n=5) 82-103% 85-96% 92-102% 81-90%