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2-AG(2-Arachidonoylglycerol) ELISA Kit
ELK8163
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48 T $265.00
96 T $376.00
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Overview

Product name: 2-AG(2-Arachidonoylglycerol) ELISA Kit
Reactivity: General
Alternative Names: 2-AG
Assay Type: Competitive Inhibition
Sensitivity: 1.41 ng/mL
Standard: 300 ng/mL
Detection Range: 4.69-300 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Neuro science
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 2-AG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 2-AG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 2-AG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
300.00 0.191
150.00 0.367
75.00 0.567
37.50 0.861
18.75 1.169
9.38 1.585
4.69 1.762
0.00 2.132

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 2-AG and the recovery rates were calculated by comparing the measured value to the expected amount of 2-AG in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 78-93% 85%
Heparin plasma(n=5) 90-105% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 2-AG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-103% 86-92% 89-103% 79-95%
EDTA plasma(n=5) 85-104% 87-106% 83-92% 82-90%
Heparin plasma(n=5) 85-96% 83-98% 88-97% 96-104%