Products

LTA4(Leukotriene A4) ELISA Kit
ELK8074
Size： Price：
48 T $265.00
96 T $376.00
Download ① Send email to us

Overview

Product name: LTA4(Leukotriene A4) ELISA Kit
Reactivity: General
Alternative Names: LT-A4
Assay Type: Competitive Inhibition
Sensitivity: 46.3 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Metabolic pathway;Infection immunity;Autoimmunity;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LTA4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LTA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LTA4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
10000.00 0.235
5000.00 0.356
2500.00 0.524
1250.00 0.913
625.00 1.253
312.50 1.572
156.25 1.799
0.00 2.325

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant LTA4 and the recovery rates were calculated by comparing the measured value to the expected amount of LTA4 in samples.
Matrix Recovery range Average
serum(n=5) 92-107% 99%
EDTA plasma(n=5) 78-92% 85%
Heparin plasma(n=5) 87-99% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LTA4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 82-96% 95-102% 82-90%
EDTA plasma(n=5) 90-99% 95-106% 87-101% 82-97%
Heparin plasma(n=5) 90-99% 90-99% 82-101% 93-105%