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8-epi-PGF2a(8-Epi Prostaglandin F2 Alpha) ELISA Kit
ELK8044
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48 T $265.00
96 T $376.00
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Overview

Product name: 8-epi-PGF2a(8-Epi Prostaglandin F2 Alpha) ELISA Kit
Reactivity: General
Alternative Names: 8-EpiPGF2-α; 8epi-PGF2a
Assay Type: Competitive Inhibition
Sensitivity: 9.55 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Pulmonology;Hepatology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-epi-PGF2a protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-epi-PGF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-epi-PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 0.191
1000.00 0.310
500.00 0.567
250.00 0.895
125.00 1.169
62.50 1.537
31.25 1.869
0.00 2.028

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 8-epi-PGF2a and the recovery rates were calculated by comparing the measured value to the expected amount of 8-epi-PGF2a in samples.
Matrix Recovery range Average
serum(n=5) 81-95% 88%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 87-99% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-epi-PGF2a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 93-102% 87-98% 91-102%
EDTA plasma(n=5) 88-97% 86-97% 85-103% 89-97%
Heparin plasma(n=5) 92-103% 83-94% 92-105% 89-97%