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Human Anti-HPF4(Anti-Heparin/Platelet Factor 4 Antibody) ELISA Kit
ELK8037
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48 T $320.00
96 T $458.00
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Overview

Product name: Human Anti-HPF4(Anti-Heparin/Platelet Factor 4 Antibody) ELISA Kit
Reactivity: Human
Alternative Names: HPF4 Ab
Assay Type: Competitive Inhibition
Sensitivity: 0.59 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 2.5h
Research Area: Hematology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-HPF4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-HPF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-HPF4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 0.215
50.00 0.275
25.00 0.596
12.50 0.842
6.25 1.139
3.13 1.585
1.57 1.887
0.00 2.157

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-HPF4 and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-HPF4 in samples.
Matrix Recovery range Average
serum(n=5) 80-97% 88%
EDTA plasma(n=5) 88-99% 93%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-HPF4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 93-102% 79-94% 87-98%
EDTA plasma(n=5) 86-93% 91-105% 87-98% 89-94%
Heparin plasma(n=5) 86-97% 93-102% 87-98% 83-97%