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Pig cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit
ELK8032
Size： Price：
48 T $412.00
96 T $588.00
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Overview

Product name: Pig cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit
Reactivity: Pig
Alternative Names: CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T; TNNT2; Troponin T Type 2, Cardiac
Assay Type: Sandwich
Sensitivity: 6 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.126 2.043
500.00 1.407 1.324
250.00 1.102 1.019
125.00 0.819 0.736
62.50 0.509 0.426
31.25 0.325 0.242
15.63 0.230 0.147
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant cTnT/TNNT2 and the recovery rates were calculated by comparing the measured value to the expected amount of cTnT/TNNT2 in samples.
Matrix Recovery range Average
serum(n=5) 78-92% 85%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 80-97% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of cTnT/TNNT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-93% 93-101% 86-97% 90-98%
EDTA plasma(n=5) 85-92% 89-96% 79-98% 81-92%
Heparin plasma(n=5) 88-97% 86-92% 94-103% 89-97%